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     Ambra Giannetti     

Ambra Giannetti joined as researcher the Institute of Applied Physics (CNR) in Florence, Italy, since 2004. She graduated in Pharmaceutical Chemistry and Technology in 2001 and received her PhD in Medical physiopathology and pharmacology in 2005, at the University of Pisa, Italy. Her research activity is focused on the study and development of optical biosensors and biosurface treatment including drugs, antibodies, enzymes, aptamers and nucleic acid probes for the preparation of active surfaces. She matured her knowledge and expertise also working abroad as result of being awarded:

    • 1 month at the Fitzpatrick Institute for Photonics, Duke University (Durham, North Carolina, USA) within the program sponsored by CNR “Short Term Mobility” (2013);
    • 1 month at the Ecologic Chemistry Institute of the Helmholtz Centre, Munich, Germany, within the program sponsored by CNR “Short Term Mobility” (2009);
    • 1 year within the “Advanced Short Term Research Opportunity Program”, at the Oak Ridge National Laboratory, Tennessee, USA, sponsored by the ORNL (USA) (2003-2004).

The achieved results during those activities and the matured experiences up to now bring her to be:

    • the Unit coordinator, for the period 2013-2016, in the project “Ultra-Sensitive Flow-Through Optofluidic MicroResonators for Biosensing Applications”, funded by the italian Ministero dell’Istruzione, dell’Università e della Ricerca (MIUR).
    • the Unit coordinator, for the period of 2012-2016, in the project “Resonant structures for the detection of biomarkers precursors of sepsis”, funded by the Centro Studi e Ricerche e Museo Storico della Fisica Enrico Fermi, Rome, Italy.
    • the Italian coordinator, for the period 2011-2013, in the Oriented Project “Novel photonic biochips for genetic applications and sepsis diagnosis”, within the European Network - Photonics for Life;
    • the Italian coordinator, in the period 2010-2012, for the bilateral project “Memory formation: effect of cholinergic system on local brain protein synthesis and its impairment by aging” presented within the bilateral program of Agreement between CNR and BAS (Bulgary).

She is Reviewer for the following international journals: Analytical and Bioanalytical Chemistry, Biosensors & Bioelectronics, ChemComm, Cytometry Part A, International Journal of Environmental Analytical Chemistry, Journal of Biophotonics, Journal of Bioenergetics and Biomembranes, Lab on a Chip.

She performed teaching activities on “optical chemical and biochemical sensors” within several national and international advanced courses and workshops. She has been part of international commissions as international expert for several PhD thesis, Madrid and Barcelona (Spain) and as external consultant for the European project WINEBIOCODE at INESC, Porto (Portugal). She has been part of several evaluating commissions for post Doctoral fellowship and contracts for temporary positions at the CNR. She participated in several European Projects (Nanodem, Hemospec, Careman, CliniChip) and in other national and international projects. She has been reviewer and referee for the evaluation of national projects such as proposals under the Italian Ministry of Economical Development (MiSE), within the program “Fondo Crescita Sostenibile”. With her research activity she contributed for publications on international refereed journals, books and conference proceedings (H-index=12, Google Scholar).

Publications List

  • International Journals

Berneschi S., Baldini F., Cosci A., Farnesi D., Nunzi Conti G., Tombelli S., Trono C., Pelli S., Giannetti A., Fluorescence biosensing in selectively photo–activated microbubble resonators, Sensors and Actuators B: Chemical 2016, In Press.

Cosci A., Berneschi S., Giannetti A., Farnesi D., Cosi F., Baldini F., Nunzi Conti G., Soria S., Barucci A., Righini G., Pelli S., Resonance frequency of optical microbubble resonators: Direct measurements and mitigation of fluctuations, SENSORS 2016, 16(9), 1405; doi:10.3390/s16091405.

Luppa PB., Bietenbeck A., Beaudoin C., Giannetti A., Clinically relevant analytical techniques, organizational concepts for application and future perspectives of point-of-care testing, BIOTECHNOLOGY ADVANCES 2016, 34, 139-160.

Cennamo N., Chiavaioli F., Trono C., Tombelli S., Giannetti A., Baldini F., Zeni L., A complete optical sensor system based on a POF-SPR platform and a thermo-stabilized flow cell for biochemical applications, SENSORS 2016, 16 (2), 196.

Aray A., Chiavaioli F., Arjmand M., Trono C., Tombelli S., Giannetti A., Cennamo N., Soltanolkotabi M., Zeni L., Baldini F., SPR-based plastic optical fibre biosensor for the detection of C-reactive protein in serum, JOURNAL OF BIOPHOTONICS 2016, DOI: 10.1002/jbio.201500315.

Adinolfi B., Pellegrino M., Giannetti A., Tombelli S., Trono C., Sotgiu G., Varchi G., Ballestri M., Posati T., Carpi S., Nieri P., Baldini F., Molecular beacon-decorated Polymethylmethacrylate Core-shell Fluorescent Nanoparticles for the Detection of Survivin mRNA in Human Cancer Cells, BIOSENSORS AND BIOELECTRONICS 2016, doi:10.1016/j.bios.2016.05.102.

Chiavaioli F, Biswas P, Trono C, Jana S, Bandyopadhyay S, Basumallick N, Giannetti A, Tombelli S, Bera S, Malick A, Baldini F., Sol-gel based titania-silica thin film overlay for long period fiber grating-based biosensors, ANALYTICAL CHEMISTRY 2015, 87 (24), pp 12024–12031

Cosci A, Quercioli F, Farnesi D, Berneschi S, Giannetti A, Cosi F, Barucci A, Nunzi Conti G, Righini G, Pelli S., Confocal reflectance microscopy for determination of microbubble resonator thickness, OPTICS EXPRESS 2015, 23, 13, 16693-16701.

A. Giannetti, A.; Barucci, A.; Cosi, F.; Pelli, S.; Tombelli, S.; Trono, C.; Baldini, F., Optical Fiber Nanotips Coated with Molecular Beacons for DNA Detection, Sensors 2015, 15, 9666-9680.

A. Barucci, A. Cosi, F.; Giannetti, A.; Pelli, S; Griffini, D.; Insinna, M.; Salvadori, S.; Tiribilli, B.; Righini, G.C., Optical fibre nanotips fabricated by a dynamic chemical etching for sensing applications, Journal of Applied Physics 2015, 117, 053104.

G. Tuci, L. Luconi, A. Rossin, F. Baldini, S. Tombelli, C. Trono, A. Giannetti, S. Cicchi, I. Manet, S. Fedeli, A. Brandi, G. Giambastiani. Hetero-bi-Functional Spacer for the Smart Engineering of Carbon-based Nanostructures. ChemPlusChem (2015), 80, 704 – 714 (Front cover article).

Barucci, A. Cosi, F.; Giannetti, A.; Pelli, S; Griffini, D.; Insinna, M.; Salvadori, S.; Tiribilli, B.; G.C. Righini, G.C., Optical fibre nanotips fabricated by a dynamic chemical etching for sensing applications. Journal of Applied Physics (2015) 117, 053104.

S. Carpi, S. Fogli, A. Giannetti, B. Adinolfi, S. Tombelli, E. Da Pozzo, A. Vanni, E. Martinotti, C. Martini, M. C. Breschi, M. Pellegrino, P. Nieri, F. Baldini. Theranostic properties of a survivin-directed molecular beacon in human melanoma cells. PLOS ONE (2014) e114588.

F. Chiavaioli, P. Biswas, C. Trono, S. Bandyopadhyay, A. Giannetti, S. Tombelli, N. Basumallick, K. Dasgupta, F. Baldini. Towards sensitive label-free immunosensing by means of turn-around point long period fiber gratings. Biosensors & Bioelectronics (2014) 60 305–310. (Impact factor 2013: 6.451).

C. Berrettoni, S. Berneschi, R. Bernini, A. Giannetti, I.A. Grimaldi, G. Persichetti, G. Testa, S. Tombelli, C. Trono, F. Baldini. Optical monitoring of therapeutic drugs with a novel fluorescence based POCT device. Procedia Engineering (2014) 87, 392–395.

Adinolfi B, Carpi S, Giannetti A, Nieri P, Pellegrino M, Sotgiu G, Tombelli S, Trono C, Varchi G, Baldini F. Complex nanostructures based on oligonucleotide optical switches and nanoparticles for intracellular mRNA sensing and silencing. Procedia Engineering 87 (2014) 751 – 754.

Lana D, Cerbai F, Di Russo J, Boscaro F, Giannetti A, Petkova-Kirova P, Pugliese AM, Giovannini MG. Hippocampal long term memory: Effect of the cholinergic system on local protein synthesis. Neurobiology of Learning and Memory (2013) 106 246–257.

A. Giannetti, S. Tombelli, F. Baldini. Oligonucleotide optical switches for intracellular sensing, Anal. Bioanal. Chem. (2013) 405(19) 6181-6196.

G.Ghini, C.Trono, A.Giannetti, G.L.Puleo, L.Luconi, J.Amadou, G.Giambastiani, F.Baldini. Carbon Nanotubes modified with fluorescein derivatives for pHnanosensing. Sensor Actuat B-Chem (2013) 179: 163-169.

Chiavaioli F, Trono C, Giannetti A, Brenci M, Baldini F. Characterisation of a label-free biosensor based on long period grating. J Biophotonics (2012) 5 (2): 1-11.

Baldini F, Brenci M, Chiavaioli F, Giannetti A, Trono C. Optical fibre gratings as tools for chemical and biochemical sensing. Anal Bioanal Chem (2012) 402: 109-16.

Surdo S, Merlo S, Carpignano F, Strambini LM, Trono C, Giannetti A, Baldini F, Barillaro G. Optofluidic microsystems with integrated vertical one-dimensional photonic crystals for chemical analysis. LAB CHIP (2012); 12: 4403-15 (Impact factor 2012: 5.697).

Giannetti A, Berneschi S, Baldini F, Cosi F, Conti GN, Soria S. Performance of Eudragit coated whispering gallery mode resonator-based immunosensors. SENSORS-BASEL (2012) 12: 14604-11.

Elisa L, Baldini F, Giannetti A, Trono C, Carofiglio T. Solid-supported Zn(II) porphyrin tweezers as optical sensors for diamines. CHEM COMMUN (2010) 46: 3678-80. (Impact factor 2010: 5.787).

Baldini F, Bolzoni L, Giannetti A, Kess M, Krämer PM, Kremmer E, Porro G, Senesi F, Trono C. A new procalcitonin optical immunosensor for POCT applications. Anal Bioanal Chem (2009) 393: 1183-90.

Baldini F, Carloni A, Giannetti A, Porro G, Trono C. An optical PMMA biochip based on fluorescence anisotropy: application to C-reactive protein assay. Sensor Actuat B-Chem. (2009) 139: 64-68.

Soria S, Baldini F, Berneschi S, Cosi F, Giannetti A, Conti GN, Pelli S, Righini GC, Tiribilli B. High-Q polymer-coated microspheres for immunosensing applications. OPT EXPRESS (2009) 17: 14694-9.

 Baldini F, Carloni A, Giannetti A, Trono C, Porro G, Tedeschi L. An optical platform based on fluorescence anisotropy for C — reactive protein assay. IEEE SENSORS (2008) 1198-1201.

Baldini F, Carloni A, Giannetti A, Porro G, Trono C. A new optical platform for biosensing based on fluorescence anisotropy. Anal Bioanal Chem (2008) 391: 1837-44.

Baldini F, Giannetti A, Mencaglia A.A, Trono C. Fiber Optic Sensors for Biomedical Applications. Curr Anal Chem (2008) 4: 378-390.

Baldini F, Carloni A, Giannetti A, Mencaglia A, Porro G, Tedeschi L, Trono C. Optical PMMA Chip Suitable for Multianalyte Detection. IEEE SENS J (2008) 8: 1305-1309.

Baldini F, Giannetti A, Mencaglia A.A. Optical sensor for interstitial pH measurements. Journal Of Biomedical Optics, (2007) 12(2), 024024.

Giannetti A, Citti L, Domenici C, Tedeschi L, Baldini F, Wabuyele M.B, Vo-Dinh T. FRET based protein-DNA binding assay for detection of active NF-kB. Sensors And Actuators. B, Chemical. (2006) 113, 649-654.

Andreu Y, Baldini F, Giannetti A, Mencaglia A. Mathematical model for the analytical signal of an herbicide f an herbicide sensor based on the reaction centre of Rhodobacter sphaeroides. TALANTA, (2005) 65, 586-592.

Baldini F, Domenici C, Giannetti A, Masci D, Mencaglia A. Time-resolved absorption as optical method for herbicide detection. Sensors And Actuators. B, Chemical. (2003) 90, 198-203.